상아모세포 분화과정에서 nuclear factor Ι-C의 역할
Role of Nuclear Factor Ι-C in Odontoblast Differentiation
배현숙, 정문진, 김흥중, 박민주, 안성민, Gronostajski, 박주철,
소속 상세정보
배현숙 ( Bae Hyun-Sook ) - 조선대학교 치과대학 구강조직학교실
정문진 ( Jeong Moon-Jin ) - 조선대학교 치과대학 구강조직학교실
김흥중 ( Kim Heung-Joong ) - 조선대학교 치과대학 구강해부학교실
박민주 ( Park Min-Ju ) - 조선대학교 치과대학 구강조직학교실
안성민 ( Ahn Seong-Min ) - 조선대학교 치과대학 구강조직학교실
( Gronostajski ) - 뉴욕 주립대학 의학 및 생의학 과학연구실
박주철 ( Park Ju-Cheol ) - 조선대학교 치과대학 구강조직학교실
KMID : 0829220050290020083
Abstract
Nuclear factor I (NFI) was discovered as a protein required for adenovirus DNA replication in vitro, but it is now clear that NFI protein plays an important role in the expression of many cellular genes. NFI-C null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and thus molar lacking roots while other tissues/organs in the body, including ameloblasts appear to be unaffected and normal. However, little is known about the mechanism of NFI-C function in odontoblast differentiation and dentin formation. In this study, in order to elucidate the molecular mechanisms of odntoblast differentiation, we examined morphological characteristics of the aberrant odontoblast in NFI-C null mice. we also evaluate the expression of dentin sialophosphoprotein (DSPP) and bone sialoprotein (BSP) mRNAs in the MDPC-23 cells by northern analysis after over-expression and inactivation of NFI-C into mouse MDPC-23 cells. Odontoblasts of the NFI-C null mouse were round in shape, lost their polarity, organized as a sheet of cells, and trapped in osteodentin-like mineralized tissue. Abnormal odontoblasts of NFI-C null mouse revealed the absence of an intercellular junctional complex known as the terminal webs. MDPC-23 cells started to express DSPP mRNA beginning from the postnatal day of 14 and showed a steady increase as differentiating into odontoblasts. Over-expression of NFI-C increased the expression of DSPP mRNA. Inactivation of NFI-C induced BSP mRNA expression. These results suggest that NFI-C plays an important role in odontoblast differentiation in a cell-type specific manner and thus in dentin formation.
키워드
NFI-C;Odontoblast differentiation;Root formation;DSPP;BSP
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